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B. P. Chavan ¹, J. R. Kadam ¹

Affiliations
1 Department of Entomology, Post Graduate Institute, Mahatma Phule Krishi Vidyapeeth, Rahuri 413722, District Ahmednagar, Maharashtra, IN

Abstract

The effect of ultraviolet irradiation on the viability of entomopathogenic fungus, Lecanicillium (=Verticillium) lecanii (Zimmerman) Zare and Gams was studied under laboratory conditions at Biocontrol Research Laboratory of Entomology, Mahatma Phule Krishi Vidyapeeth, Rahuri, Maharashtra during 2002-04. The liquid formulation of L. lecanii with different adjuvants, viz., glycerol, Tween-80, Triton-x-100, boric acid, indigo, milk, turmeric and arachid oil were exposed to UV light for different exposure periods of 10, 20, 30, 40 and 50 min. The distance between exposed suspension and UV light source was 0.3 m. The UV rays proved detrimental to the growth and development of L. lecanii. The detrimental effect increased with increase in exposure period. However, glycerol, boric acid and Tween-80 gave good UV protection to L. lecanii, while milk, turmeric and indigo lack the UV protectability.

Keywords

Adjuvant, UV Light, Lecanicillium lecanii, Viability.

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B. P. Chavan ¹, J. R. Kadam ¹

Affiliations
1 Department of Entomology, Post-Graduate Institute, Mahatma Phule Krishi Vidyapeeth, Rahuri 413722, District Ahmednagar, Maharashtra, IN

Abstract

Effect of some adjuvant combinations on the growth of Verticillium lecanii (Zimmermann) Viegas in culture medium and the subsequent infection of Maconellicoccus hirsutus (Green) were evaluated at Mahatma Phule Krishi Vidyapeeth, Rahuri, Maharashtra, during 2002-04. The treatment including V. l. + glycerol (8%) + Tween 80 (1%) + arachid oil (5%) proved to be the most promising, recording maximum surface coverage (92.00%) and biomass (32.10g) at 10 days of inoculation and bioefficacy at 14 days. However, it was on par with the formulations containing V. l. + glycerol 5% + Tween 80 1% + arachid oil 2% and V. l. + glycerol 2% + Tween 80 1% + arachid oil 0.5%. In terms of viability and virulence, V. l. + glycerol 2% + Tween 80 1% + arachid oil 0.5% and V. l. + glycerol 5% + Tween 80 1% + arachid oil 2% emerged as the best combinations.

Keywords

Adjuvants, Arachid Oil, Glycerol, Maconellicoccus hirsutus, Tween-80, Verticillium lecanii Viability and Virulence.

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B. P. Chavan ¹, J. R. Kadam ¹

Affiliations
1 Department of Entomology, Post Graduate Institute, Mahatma Phule Krishi Vidyapeeth, Rahuri 413722, Ahmednagar, Maharashtra, IN

Abstract

Liquid formulations of entomopathogenic fungus, Verticillium lecanii (Zimmermann) Viegas were evaluated against spiralling whitefly, Aleurodicus dispersus Russell at Biocontrol Research Laboratory, Mahatma Phule Krishi Vidyapeeth, Rahuri, during 2002-04. The study revealed that liquid formulation A (VGTA 50512) and B (VGTA 50510-5) of V. lecanii caused 78.33 to 92.34 and 80.20 to 93.45 per cent mortality of the whitefly, respectively. V. lecanii at one per cent concentration gave the highest mortality of 92.34 per cent (Formulation A) and 93.45 per cent (Formulation B) respectively but it was on par with the concentration of 0.45 to 0.75 per cent. So, 0.45 per cent concentration of the liquid formulation is the most economical and feasible concentration for the control of the pest.

Keywords

Aleurodicus dispersus, Bioefficacy, Liquid Formulation and Verticillium lecanii.

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B. P. Chavan ¹, J. R. Kadam ²

Affiliations
1 Department of Entomology, Post Graduate Fnstitute, Mahatma Phule Krishi Vidyapeeth, Rahuri, 413 722, Dist. Ahmednagar, Maharashtra, IN
2 Department of Entomology, Post Graduate Institute, Mahatma Phule Krishi Vidyapeeth, Rahuri, 413 722, Dist. Ahmednagar, Maharashtra, IN

Abstract

Studies on the effect of various adjuvants on growth and development of Verticillium lecanii (Zimmermann) Viegas was undertaken with a view to select suitable adjuvant for developing liquid formulation. The study revealed that there was increase in surface area covered (%) and biomass produced (gm) with increase in concentration of the inoculum. Among the different oils tested, significant surface area coverage was recorded in arachid oil @ 1.0 per cent followed by 0.1 to 0.5 per cent, respectively. Similar result was recorded in biomass production. Addition of arachid oil @ 1.0 per cent recorded maximum biomass of 28.40 g, followed by 0.5 per cent (25.2 g) and 0.1 per cent (23.5 g), respectively. Addition of oils and stickers resulted in increased surface area growth significantly. Addition of arachnid oil @ 5 per cent resulted in maximum surface area of biomass. Based on the results it is concluded that arachid oil could be used as adjuvant in the liquid formulation of V. lecanii isolated from spiraling whitefly.

Keywords

Bioefficacy, Growth And Development, Maconelicoccus hirsutus, Verticillium lecanii.

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